Enzo ALX-805-041-C100 CD45 (human) monoclonal antibody (MEM-28) (100 µg)
| Application | Flow Cytometry, ICC, IHC (PS), IP, WB |
|---|---|
| Clone | MEM-28 |
| Formulation | Liquid. In PBS, pH 7.4, containing 15mM sodium azide. |
| Host | Mouse |
| Immunogen | Human thymocytes and T lymphocytes. |
| Isotype | IgG1 |
| Purity Detail | Protein A-affinity purified. |
| Recommendation Dilutions/Conditions | Flow Cytometry (1µg/ml)Immunocytochemistry (10µg/ml, paraformaldehyde fixation can be used)Immunohistochemistry (paraffin sections, no pre-treatment of tissue sections is essential)Western Blot (1µg/ml; Positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2x non-reducing SDS.Application note: non-reducing conditions. SDS-PAGE (6% separating gel))Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Species Reactivity | Human |
| Specificity | Recognizes all isoforms of CD45. |
| UniProt ID | P08575 |
| Worry-free Guarantee | This antibody is covered by our Worry-Free Guarantee. |
| Handling | Avoid freeze/thaw cycles. |
| Long Term Storage | +4°C |
| Shipping | Blue Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352203 |