Enzo ALX-850-014-KI02 FasL, soluble (human) (recombinant) set (1 Set)

APO-1L, CD95L, CD178, TNFSF 6

FUNC

Kills Fas-sensitive cells at concentrations of >1ng/ml in the presence of cross-linking enhancer.

FasL: 0.1mg/ml after reconstitutionEnhancer: 1mg/ml

10µg of FasL, Soluble (human) (recombinant) (Prod. No. ALX-522-001) and 4×50µg of Enhancer for Ligands (Prod. No. ALX-804-034) which increases the biological activity (induction of apoptosis) by ~50-fold to a concentration range of 1-5ng/ml.

FasL: Lyophilized. Contains PBS.
Enhancer: Liquid. In PBS, pH7.4, containing 50% glycerol. Contains no preservatives.

≥95% (SDS-PAGE)

Reconstitute FasL with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.

Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG^®-tag. Glycosylation of FasL, Soluble (human) (recombinant) is similar to natural human FasL.

ED₅₀: 1ng/ml (A20 cells)

FasL binds to human, mouse and rat Fas (CD95).

FLAG is a registered trademark of Sigma-Aldrich Co.

P48023

Stable for at least 6 months after receipt when stored at -20°C.

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

-20°C

Blue Ice