Mfg Part Number
ALX-850-018-KI01

Enzo ALX-850-018-KI01 TRAIL, soluble (human) (recombinant) set (1 Set)

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Enzo ALX-850-018-KI01 TRAIL, soluble (human) (recombinant) set (1 Set)
$815.36
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Alternative Name

Apo-2L, TNFSF 10, CD253

Application

FUNC

Application Notes

Induces apoptosis in a concentration range of 1-100ng/ml if applied with the cross-linking enhancer.

Concentration

TRAIL: 0.1mg/ml after reconstitutionEnhancer: 1mg/ml after reconstitution

Contents

10µg of TRAIL, Soluble (human) (recombinant) (Prod. No. ALX-522-003) and 2×50µg of Enhancer for Ligands (Prod. No. ALX-804-034).

Formulation

Lyophilized. Contains PBS.

Purity

≥95% (SDS-PAGE)

Reconstitution

Reconstitute TRAIL with 100µl and each vial of enhancer with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

Source

Produced in E. coli. The extracellular domain of human TRAIL (Apo-2L) (aa 95-281) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.

Specific Activity

ED50: 10ng/ml (Jurkat cells)

Specificity

TRAIL binds to human and mouse TRAIL receptors and human osteoprotegerin (OPG).

Technical Info / Product Notes

FLAG is a registered trademark of Sigma-Aldrich Co.

UniProt ID

P50591

Use/Stability

Stable for at least 6 months after receipt when stored at -20°C.

Handling

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Long Term Storage

-20°C

Shipping

Blue Ice

More Information
Brand Enzo
UNSPSC 12352202