Enzo ALX-850-018-KI01 TRAIL, soluble (human) (recombinant) set (1 Set)
| Alternative Name | Apo-2L, TNFSF 10, CD253 |
|---|---|
| Application | FUNC |
| Application Notes | Induces apoptosis in a concentration range of 1-100ng/ml if applied with the cross-linking enhancer. |
| Concentration | TRAIL: 0.1mg/ml after reconstitutionEnhancer: 1mg/ml after reconstitution |
| Contents | 10µg of TRAIL, Soluble (human) (recombinant) (Prod. No. ALX-522-003) and 2×50µg of Enhancer for Ligands (Prod. No. ALX-804-034). |
| Formulation | Lyophilized. Contains PBS. |
| Purity | ≥95% (SDS-PAGE) |
| Reconstitution | Reconstitute TRAIL with 100µl and each vial of enhancer with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
| Source | Produced in E. coli. The extracellular domain of human TRAIL (Apo-2L) (aa 95-281) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
| Specific Activity | ED50: 10ng/ml (Jurkat cells) |
| Specificity | TRAIL binds to human and mouse TRAIL receptors and human osteoprotegerin (OPG). |
| Technical Info / Product Notes | FLAG is a registered trademark of Sigma-Aldrich Co. |
| UniProt ID | P50591 |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Handling | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| Long Term Storage | -20°C |
| Shipping | Blue Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352202 |