Functional Application (blocks immune complex binding, as does the F(ab’)2 fragment). The F(ab’)2 fragment blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII receptors in vitro. It is therefore a useful tool to reduce strong background staining of Fc-containing MAbs in FC and IHC. Also useful when recombinant Fc:fusion proteins are employed as detection tools for the detection of the cognate ligands.
Clone
7.3
Host
Mouse
Immunogen
Human K562 tumor cells and L cells transfected with human CD32 (FcγRII).
Isotype
IgG1
Species Reactivity
Human
Specificity
Recognizes an epitope in domain 2 of all CD32 isoforms.
