Enzo BML-AK302-0001 Matrix metalloproteinase-2 (MMP-2) fluorometric drug discovery kit, RED (96 wells)

Gelatinase A, 72 kDa Type IV collagenase

Activity assay, Fluorescent detection, HTS

Designed to screen MMP-2 inhibitors using a quenched fluorogenic peptide.

1 vial MMP-2 enzyme
1 vial substrate (OMNIMMP^® RED)
1 vial 6′-TAMRA calibration standard
1 vial control inhibitor (NNGH)
1 bottle (20 ml) assay buffer
1 black 96-well microplate
Instructions

NCBI Reference Sequence: NM_004530

The OMNIMMP^® RED substrate offers key advantages over other MMP substrates.

1. Emission at the red end of the spectrum (576 nm after excitation at 545 nm) avoids the interference at lower wavelengths often exhibited by screening compounds, and by substances commonly found in biological samples and tissue culture medium.
2. MMP substrate peptides display poor aqueous solubility, often with K_ms near their limits of solubility, making enzyme and inhibitor kinetics difficult.  MMP K_ms for OMNIMMP^® RED substrate are below its solubility limit.

In addition to the efficient binding as exhibited by low K_ms, OMNIMMP^® RED is avidly cleaved by MMPs, with k_cat/K_ms in the range of 10⁴-10⁶ M^-1sec^-1.

3. The ultra-strong fluorescence of OMNIMMP^® RED allows for substrate concentrations much lower than the K_m, a condition generally desirable in inhibitor screening assays.

P08253

Avoid freeze/thaw cycles.

-80°C

Dry Ice