Enzo BML-AK308-0001 Matrix metalloproteinase (MMP) inhibitor profiling kit, fluorometric RED (96 wells)

Activity assay, Fluorescent detection, HTS

Designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes, using a quenched fluorogenic peptide.

10 vials (10 MMP enzymes)
1 vial Substrate (OMNIMMP^® RED)
1 vial 6′-TAMRA calibration standard
1 vial control inhibitor (NNGH)
2 bottles (20 ml each) assay buffer
1 black 96-well microplate
Instructions

NCBI Reference Sequence: NM_002429, NM_004530, NM_002422, NM_002423, NM_002424, NM_004994, NM_002425, NM_002426, NM_002427, NM_004995

The OMNIMMP^® RED substrate offers key advantages over other MMP substrates.

1. Emission at the red end of the spectrum (576 nm after excitation at 545 nm) avoids the interference at lower wavelengths often exhibited by screening compounds, and by substances commonly found in biological samples and tissue culture medium.
2. MMP substrate peptides display poor aqueous solubility, often with K_ms near their limits of solubility, making enzyme and inhibitor kinetics difficult.  MMP K_ms for OMNIMMP^® RED substrate are below its solubility limit.
3. In addition to the efficient binding as exhibited by low K_ms, OMNIMMP^® RED is avidly cleaved by MMPs, with k_cat/K_ms in the range of 10⁴-10⁶ M^-1sec^-1.
4. The ultra-strong fluorescence of OMNIMMP^® RED allows for substrate concentrations much lower than the K_m, a condition generally desirable in inhibitor screening assays.

Avoid freeze/thaw cycles.

-80°C

Dry Ice