Enzo BML-AK312-0001 Matrix metalloproteinase-12 (MMP-12) fluorometric drug discovery kit, GREEN (96 wells)

Metalloelastase, Macrophage elastase

Activity assay, Fluorescent detection, HTS

Designed to screen MMP-12 inhibitors using a quenched fluorogenic substrate.

1 vial MMP-12 enzyme
1 vial substrate (MMP-3 fluorogenic substrate)
1 vial 5-FAM calibration standard
1 vial control inhibitor (NNGH)
1 bottle (20 ml) assay buffer
1 black 96-well microplate
Instructions

NCBI Reference Sequence: NM_004994
The green fluorogenic MMP substrate provided in the kit offers key advantages over other MMP substrates. 1) Emission in the green region of the spectrum avoids the interference at lower wavelengths often exhibited by screening compounds, and by substances commonly found in biological samples and tissue culture medium. 2) The ultra-strong fluorescence of this substrate allows for substrate concentrations much lower than the K_m, a condition generally desirable in inhibitor screening/kinetics assays.

P39900

Avoid freeze/thaw cycles.

-80°C

Dry Ice