Enzo BML-AK511-0001 FLUOR DE LYS® HDAC1 fluorometric drug discovery assay kit (96 wells)

Histone deacetylase 1 fluorescent assay kit

Activity assay, Cell-based assays, Fluorescent detection, HTS

HDAC1 (Histone Deacetylase 1) (human, recombinant) (Prod. No. (BML-SE456)
(50 µg; 10mM TRIS, pH 7.5, 100mM sodium chloride, 3mM magnesium chloride, 10% glycerol)
Storage: -80°C, avoid freeze/thaw cycles
FLUOR DE LYS^® SIRT1, Deacetylase Substrate (Prod. No. BML-KI177)
(100µl; 5mM solution in 50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride)
Storage: -80°C
FLUOR DE LYS^® Developer II Concentrate (5x) (Prod. No. BML-KI176)
(5 x 250 µl; 5x Stock Solution; Dilute in Assay Buffer before use)
Storage: -80°C
Trichostatin A (HDAC Inhibitor) (Prod. No. BML-GR-309-9090)
(100 µl; 0.2mM in DMSO)
Storage: -80°C
FLUOR DE LYS^® Deacetylated Standard (Prod. No. BML-KI142)
(30 µl; 10mM in DMSO)
Storage: -80°C
HDAC Assay Buffer II (50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride, 1mg/ml bovine serum albumin) (Prod. No. BML-KI422)
(20 ml)
Storage: -20°C (or -80°C)
HDAC Assay Buffer (50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride) (Prod. No. BML-KI143)
(20 ml)
Storage: -20°C (or -80°C)
1/2 Volume Microplates (Prod. No. 80-2407)
1 clear and 1 white, 96-well
Storage: Ambient

Q13547

Store all components except the microplate and instruction booklet at -80°C for the highest stability. The HDAC1 Enzyme (Prod. No. BML-SE456), must be handled with particular care in order to retain maximum enzymatic activity. Defrost it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be refrozen quickly, by placing at -80°C. If possible, snap freeze in liquid nitrogen or a dry ice/ethanol bath. To minimize the number of freeze/thaw cycles, aliquot the enzyme into separate tubes and store at -80°C.

-80°C

Dry Ice