Enzo BML-AK512-0001 FLUOR DE LYS®-Green HDAC2 fluorometric drug discovery assay kit (96 wells)

Histone deacetylase 2 fluorescent assay kit

Activity assay, Cell-based assays, Fluorescent detection, HTS

Study of enzyme kinetics, modulator activity, drug discovery.

HDAC2 (Histone Deacetylase 2) (human, recombinant) (Prod. No. BML-KI575)
(67μl; 30ng/μl in 50mM TRIS, pH 8.0, 138mM NaCl, 10% glycerol, 1mg/ml BSA )
Storage: -70°C. Avoid freeze/thaw cycles!
FLUOR DE LYS^®-Green substrate (Prod. No. BML-KI572)
(50μl; 50mM in DMSO)
Storage: -70°C
FLUOR DE LYS^® Developer concentrate (20x) (Prod. No. BML-KI105)
(300μl; 20x Stock solution; Dilute in Assay Buffer before use.)
Storage: -70°C
Trichostatin A (HDAC Inhibitor) (Prod. No. BML-GR309-9090)
(100μl; 0.2mM in DMSO)
Storage: -70°C
FLUOR DE LYS^®-Green Standard (Prod. No. BML-KI605)
(30µl 1mM in DMSO)
Storage: -70°C.
Note: BML-KI605 is an improved replacement of BML-KI573.
HDAC Assay Buffer II (Prod. No. BML-KI422)
(20ml; 50mM TRIS/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl₂, 1mg/ml BSA)
Storage: -70°C
1/2 volume black NBS microplate (Prod. No. BML-KI570)
Storage: Room temperature
1/2 volume white NBS microplate (Prod. No. BML-KI571)
Storage: Room temperature

96 assays

The original FLUOR DE LYS^® HDAC Fluorescent Activity Assay revolutionized HDAC and sirtuin screening by providing a highly sensitive, mix-and-read alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of lysine deacetylases. The FLUOR DE LYS^®-Green assay has those same advantages, but also, due to its higher wavelength excitation and emission, avoids interference by quenching or fluorescence from compounds absorbing and/or emitting in the near UV and blue. FLUOR DE LYS^®-Green is an especially sensitive substrate for HDAC2.

Q92769

Store all components except the microtiter plate and instruction booklet at -70°C for the highest stability. HDAC2 (Prod. No. BML-KI575) must be handled with particular care in order to retain maximum enzymatic activity. Defrost it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be refrozen quickly, by placing at -70°C. If possible, snap freeze in liquid nitrogen or a dry ice/ethanol bath. To minimize the number of freeze/thaw cycles, aliquot the enzyme into separate tubes and store at -70°C. The FLUOR DE LYS®-Green Substrate (Prod. No. BML-KI572), when diluted in Assay Buffer, may precipitate after freezing and thawing. It is best, therefore, to dilute only the amount needed to perform the assays of that day.

-80°C

Dry Ice