Enzo BML-AK516-0001 FLUOR DE LYS® HDAC6 fluorometric drug discovery kit (96 wells)

Histone deacetylase 6 fluorescent assay kit

Activity assay, Fluorescent detection, HTS

HDAC6 (Histone Deacetylase 6) (human, recombinant) (Prod. No. BML-SE508)
(50 µg; 10mM TRIS, pH 7.5, 100mM NaCl, 3mM MgCl2, 10% glycerol)
Storage: -80°C, avoid freeze/thaw cycles!
FLUOR DE LYS^® SIRT1, Deacetylase Substrate (Prod. No. BML-KI177)
(100µl; 5mM solution in 50mM TRIS/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2)
Storage: -80°C
FLUOR DE LYS^® Developer II Concentrate (5x) (Prod. No. BML-KI176)
(5 x 250 µl; 5x Stock Solution; Dilute in Assay Buffer before use
Storage: -80°C
Trichostatin A (HDAC Inhibitor) (Prod. No. BML-GR309-9090)
(100 µl; 0.2mM in DMSO)
Storage: -80°C
FLUOR DE LYS^® Deacetylated Standard (Prod. No. BML-KI142)
(30 µl; 10mM in DMSO)
Storage: -80°C
HDAC Assay Buffer II (Prod. No. BML-KI422)
(20ml; 50mM TRIS/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2, 1mg/ml BSA)
Storage: -20°C
HDAC Assay Buffer (Prod. No. BML-KI143)
(20ml; 50mM TRIS/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2)
Storage: -20°C
1/2 volume microplate (Prod. No. BML-KI101)
Storage: Ambient
1/2 volume white NBS microplate (Prod. No. BML-KI584)
Storage: Ambient

Q9UBN7

Store all components except the microplate at -80°C for the highest stability. The HDAC6 Enzyme (Prod. No. BML-SE508), must be handled with particular care in order to retain maximum enzymatic activity. Defrost it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be refrozen quickly, by placing at -80°C. If possible, snap freeze in liquid nitrogen or a dry ice/ethanol bath. To minimize the number of freeze/thaw cycles, aliquot the enzyme into separate tubes and store at -80°C.

-80°C

Dry Ice