Enzo BML-AK530-0001 FLUOR DE LYS®-Green HDAC fluorometric activity assay kit (96 wells)

Histone deacetylase green fluorescent assay kit

Activity assay, Cell-based assays, Fluorescent detection, HTS

Nuclear Extract from HeLa Cells (human cervical cancer cell line) (Prod. No. BML-KI140)
(100µl in 0.1M potassium chloride, 20mM HEPES/NaOH, 20% (v/v) glycerol, 0.2mM EDTA, 0.5mM DTT, 0.5mM PMSF)
Storage: -70°C.
FLUOR DE LYS^®-Green Substrate (Prod. No. BML-KI572)
(50µl 50mM in DMSO)
Storage: -70°C.
FLUOR DE LYS^® Developer Concentrate (20X) (Prod. No. BML-KI105)
(300µl 20x stock solution; dilute in assay buffer before use)
Storage: -70°C.
Trichostatin A (Prod. No. BML-GR309)
(100µl 0.2mM in DMSO)
Storage: -70°C.
FLUOR DE LYS^®-Green Standard (Prod. No. BML-KI605)
(30µl 1mM in DMSO)
Storage: -70°C.
Note: BML-KI605 is an improved replacement of BML-KI573.
NAD⁺ (Sirtuin Substrate) (Prod. No. BML-KI282)
(500µl; 50mM in 50mM TRIS, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride)
Storage: -70°C.
Nicotinamide (Prod. No. BML-KI283)
(500µl; 50mM in 50mM TRIS, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride)
Storage: -70°C.
HDAC Assay Buffer (Prod. No. BML-KI143)
(20ml; 50mM TRIS, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride)
Storage: -70°C.
96 Well Plates (Prod. No. BML-KI101)
Storage: Room temperature
White NBS Microplate (Prod. No. BML-KI571)
Storage: Room temperature

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Store all components (except microtiter plates) at -70°C. Unused HeLa Nuclear Extract should be refrozen quickly, if possible snap freeze in liquid nitrogen.

-80°C

Dry Ice