Enzo BML-AK531-0001 FLUOR DE LYS® HDAC3/NCOR1 fluorometric drug discovery kit (96 wells)

Histone deacetylase 3/Nuclear receptor corepressor 1 fluorometric drug discovery kit

Activity assay, Cell-based assays, Fluorescent detection, HTS

HDAC3/NCOR1 Complex (human, recombinant) (Prod. No. BML-KI574)
67 µl; 30 ng/µl in 50mM TRIS, pH 8.0, 138mM NaCl, 10% glycerol, 1 mg/ml BSA.
Storage: -80°C; avoid freeze/thaw cycles!

FLUOR DE LYS^®−SIRT1, Deacetylase Substrate (Prod. No. BML-KI177)
100 µl; 5mM solution in 50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride.
Storage: -80°C

FLUOR DE LYS^® Developer II Concentrate (5x) (Prod. No. BML-KI176)
5 x 250 µl; 5x Stock Solution; dilute in assay buffer before use.
Storage: -80°C

Trichostatin A (HDAC Inhibitor) (Prod. No. BML-GR309-9090)
100 µl; 0.2mM in DMSO
Storage: -80°C

FLUOR DE LYS^® Deacetylated Standard (Prod. No. BML-KI142)
30µl; 10mM in DMSO
Storage: -80°C

HDAC Assay Buffer II (Prod. No. BML-KI422)
20 ml; (50 mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride, 1mg/ml BSA).
Storage: -20°C

HDAC Assay Buffer (Prod. No. BML-KI143)
20 ml; (50 mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride).
Storage: -20°C

1/2 volume microplate (Prod. No. BML-KI101)
Storage: Ambient

1/2 volume white NBS microplate (Prod. No. BML-KI571)
Storage: Ambient

O15379

Store all components, except the microplate, at -80°C for the highest stability. The HDAC3/NCOR1 Complex, Prod. No. BML-KI574, must be handled with particular care in order to retain maximum enzymatic activity. Defrost it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be refrozen quickly, by placing at -80°C. If possible, snap freeze in liquid nitrogen or a dry ice/ethanol bath. To minimize the number of freeze/thaw cycles, aliquot the enzyme into separate tubes and store at -80°C.

-80°C

Dry Ice