Enzo BML-GC3008-0100 Glutamic acid decarboxylase polyclonal antibody (100 µl)

GAD

ELISA, IHC, WB

Detects two isoforms of ~65kDa and ~67 kDa by Western Blot.

Liquid. In PBS containing 0.09% sodium azide.

Rabbit

Synthetic peptide corresponding to aa 572-585 (D572FLIEEIERLGQDL585) of rat glutamate decarboxylase (GAD65). This sequence is conserved in rat GAD67 and in human GAD-2.

Protein A-affinity chromatography.

Purified from rabbit serum.

Cat, Human, Mouse, Rat

A synthetic peptide with the amino acid sequence: [C]-D-F-L-I-E-E-I-E-R-L-G-Q-D-L was selected from rat glutamate decarboxylase (GAD65; C-terminus residues [Cys]+572-585). This sequence is conserved in rat GAD67 and in human GAD-28. The peptide was conjugated to keyhole limpet hemocyanin via the N-terminal cysteine residue using m-maleimidobenzoic acid N-hydroxysuccinimide. The conjugate was used to immunise New Zealand White rabbits and the resultant antiserum (B4;Z00257) has been extensively characterised by ELISA, Western blot and immunohistochemical techniques. Application data Western blotting Test tissues: Mouse and rat brain. Dilution: 1:1000. Protein size: Doublet on SDS-PAGE at approximately 65/67kDa. Immunohistochemistry Test tissues: Human, cat and rat brain (esp. cerebellum). Fixatives: Several fixative solutions may be used. Aldehyde-combination fixatives (i.e. those containing formaldehyde and glutaraldehyde) usually give satisfactory results. Bouin and Susa fixatives containing 0.1-0.2% glutaraldehyde have been used satisfactorily. Processing: Cryostat and Vibratome®9 sections have been used most commonly. Paraffin wax-embedded tissue has been used successfully. The antiserum has also been used for the ultrastructural detection of GAD using pre-embedding immunocytochemistry. Dilution range: 1:200-1:5000, using overnight incubation and PAP or ABC-peroxidase procedure.

Q05683

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Avoid freeze/thaw cycles.

-20°C

Blue Ice