Enzo BML-KI307-5000 5'-Nucleotidase (from Crotalus atrox venom) (5000 kU)

One U will release 1pmol phosphate per minute from 200µM 5’-AMP, 30°C in a reaction buffer of 10mM Tris-HCl, pH 7.4, 0.2mM MgCl2.

5’-nucleotidase is useful as the second enzyme in coupled assays of phosphodiesterases (3’,5’-cyclic nucleotide phosphodiesterase; EC 3.1.4.17). Phosphodiesterase catalyzed hydrolysis of, for example, cAMP to 5’-AMP is coupled to 5’-nucleotidase cleavage of 5’-AMP to inorganic phosphate and adenosine. Phosphodiesterase activity is then quantified by colorimetric detection of the inorganic phosphate with a reagent such as BIOMOL Green (Prod. No. BML-AK111; see also Cyclic Nucleotide Phosphodiesterase Assay Kit, Prod. No. BML-AK800).

Liquid. In TRIS-HCl, pH 7.4, 150mM sodium chloride, 1mM magnesium chloride and 10% (v/v) glycerol.

5000kU in 1000µl

As indicated on product label or CoA when stored as recommended. Stable for at least 6 freeze-thaw cycles, but care should be taken that both freezing and thawing steps are done quickly (e.g. liquid N2 or dry ice/EtOH for freezing, rapid finger-rubbing for thawing). Frozen storage of smaller aliquots is recommended if more than 6 freeze-thaws of the entire vial contents (1000µl) are anticipated. Thawed enzyme is stable on ice for at least 2 hrs.

-80°C

Dry Ice