Enzo BML-PW0605-0100 Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4) (HRP conjugate) (100 µg)

WB

K⁶³-linked polyubiquitin modified proteins comprise only a proportion of total cellular polyubiquitinylated species. Increased sample loading may therefore be required for analysis by Western blotting compared to equivalent experiments using general ubiquitin antibodies, despite the enhanced detection capability of the HWA4C4 HRP conjugated antibody.

HWA4C4

Does not cross-react with any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitinylated species or free ubiquitin.

Liquid. In 1X PBS, pH 7.2.

Mouse

Synthetic peptide containing the ubiquitin K63-isopeptide motif.

IgG2a

Protein G-affinity purified.

Western Blot (1:1,000)Specificity for K63-isopeptide-linked polyubiquitin conjugates is temperature sensitive. Western blot membranes MUST NOT be autoclaved and excessive heat should avoided.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Purified from hybridoma tissue culture supernatant.

Species independent

Recognizes K⁶³-linked polyubiquitin in wide range of species.

P0CG47 (UBB), P0CG48 (UBC)

This antibody is covered by our Worry-Free Guarantee.

Avoid freeze/thaw cycles.

+4°C

-20°C

Blue Ice