Enzo BML-PW0930-0100 Ubiquitin monoclonal antibody (P4D1) (100 µg)

IHC, IP, WB

Clone P4D1 has been reported to be of utility in immunoprecipitation, immunofluorescence and immunohistochemistry (see Brady et al. (2005); Wang et al. (2008); Ito et al. (2003) respectively).

P4D1

Liquid. In PBS containing 0.01% sodium azide.

Mouse

Denatured bovine ubiquitin.

IgG1

Protein G affinity purified form cell culture media.

Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Purified from hybridoma tissue culture supernatant.

Species independent

Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and free ubiquitin.

The ubiquitin antibody BML-PW0930 (clone P4D1) was developed using a denatured glutaraldehyde cross-linked ubiquitin IgG complex as the immunogen. Monoclonal antibody was purified from tissue culture supernatant by ion exchange chromatography. The general ubiquitin reactivity of the monoclonal antibody has been demonstrated by Western blotting using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in cell lysates.

P0CG53 (UBB), P0CH28 (UBC), P62992 (RPS27A), P63048 (UBA52)

This antibody is covered by our Worry-Free Guarantee.

Stable for at least 1 year after receipt when stored at -20°C.

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

-20°C

-20°C

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