Enzo BML-PW0935-0100 Ubiquitin monoclonal antibody (P4D1) (HRP conjugate) (100 µl)

WB

P4D1

Liquid. In PBS containing 0.01% sodium azide.

Mouse

Denatured bovine ubiquitin.

IgG1

Ion exchange purified.

Western Blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Purified from tissue culture supernatant. HRP conjugated chemically in vitro.

Species independent

Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and free ubiquitin.

P4D1 antibody was developed using a denatured glutaraldehyde cross-linked ubiquitin IgG complex as the immunogen. Monoclonal antibody was purified from tissue culture supernatant by ion exchange chromatography. The general ubiquitin reactivity of the monoclonal antibody has been demonstrated by Western blotting using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in cell lysates.

P0CG53 (UBB), P0CH28 (UBC), P62992 (RPS27A), P63048 (UBA52)

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Avoid freeze/thaw cycles.

+4°C

+4°C

Blue Ice