Mfg Part Number
BML-PW8100-0025

Enzo BML-PW8100-0025 Proteasome 20S α6 subunit monoclonal antibody (MCP20) (25 µl)

Quick Overview
Enzo BML-PW8100-0025 Proteasome 20S α6 subunit monoclonal antibody (MCP20) (25 µl)
$175.76
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Alternative Name

PROS30, Proteasome subunit α type-1, Macropain subunit C2, Proteasome ν chain

Application

IHC, IP, WB

Application Notes

Immunoprecipitation: This antibody has been immobilised on CNBr-activated Sepharose CL-4B and used to precipitate proteasomes from HeLa cells. Proteasomal subunits were subsequently separated by 2D PAGE.
Western Blot: Single dimension SDS-PAGE of a human placental proteasome preparation or HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of approximately 33kDa.

Clone

MCP20

Crossreactivity

Does not cross-react with rat or mouse protein.

Formulation

Liquid. In PBS containing 10mM sodium azide.

Gene/Protein Identifier

PSMA1 (gene name)

Host

Mouse

Immunogen

Human placenta derived proteasomes.

Isotype

IgG1

Purity Detail

Protein G affinity purified.

Source

Purified from ascites.

Species Reactivity

Human, Rabbit

Specificity

Recognizes the α6 subunit of proteasome 20S.

Technical Info / Product Notes

Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from “standard” nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.

UniProt ID

P25786

Worry-free Guarantee

This antibody is covered by our Worry-Free Guarantee.

Use/Stability

Aliquot undiluted antibody into smaller volumes (not less than 10µL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-mouse IgG linker antibody is to be used).

Handling

Avoid freeze/thaw cycles.

Long Term Storage

-20°C

Shipping

Blue Ice

More Information
Brand Enzo
UNSPSC 12352203