Enzo BML-PW8155-0100 Proteasome 20S core subunits polyclonal antibody (100 µl)

IHC (FS), IP, WB

Western blot – The antibody has been characterised by single dimension SDS-PAGE using both purified human and yeast 20S proteasome and a number of tissue/cell preparations including a human placental proteasome preparation, a HeLa cell lysate, a RSV 3T3 mouse fibroblast cell lysate, and a yeast whole cell extract. Western blotting shows bands attributable to ‘core’ subunits at ~25-30kDa as shown opposite. Differences in subunit band intensity are noted between the samples. 2-D SDS-PAGE followed by Western blotting of purified 20S proteasome shows the presence of five/six spots with the following proteasome subunit correlation: α5/α7, β1, β5, β5i, β7. The 2-D patterns observed with human and mouse derived materials are not identical with some differences being observed in the molecular weights and isoelectric points of some subunits.

Liquid. In PBS containing 10mM sodium azide.

Rabbit

Human erythrocyte-derived proteasomes.

From rabbit serum.

Human, Mouse, Rabbit, Rat, Yeast

Recognizes the ‘core’ subunits of the 20S proteasome.

This antibody is covered by our Worry-Free Guarantee.

Dilute with PBS pH 7.2 – 7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).

Avoid freeze/thaw cycles. Aliquot undiluted antibody into smaller volumes (not less than 10µl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +2-4°C (do not freeze) and use within 1 month.

-20°C

Blue Ice