Enzo BML-PW8770-0025 Proteasome 19S Rpt5/S6a subunit monoclonal antibody (TBP1-19) (25 µl)

TBP1, Tat-binding protein 1, 26S protease regulatory subunit 6A, Proteasome 26 S subunit ATPase 3

IHC, WB

TBP1-19

Liquid. Contains 10mM sodium azide.

Mouse

Recombinant full-length human Rpt5.

IgG2b

Partially purified ascites.

Purified from hybridoma tissue culture supernatant.

Human, Mouse, Rabbit, Rat

Recognizes the Rpt5/S6a subunit of the 19S regulatory subunit

The hybridoma secreting the antibody to subunit Rpt5/S6a was generated by fusion of splenocytes from Balb/c mice that had received repeated immunisation with a full length human recombinant protein with Sp2/0-Ag14 myeloma cells. The antibody (clone TBP1-19) has been characterised by one-dimensional Western blotting.

Immunoblotting – Single dimension SDS-PAGE of a purified rabbit 26S proteasome preparation followed by Western blotting at optimal dilution (1:2500) gives a major band with a relative molecular weight of approximately 50kDa. There is a minor band observed running slightly slower that may be due to modification.
Immunoprecipitation – This antibody has not been characterised for this purpose.
Immunohistochemistry – This antibody has been shown to be suitable for this purpose in the subcellular localisation of proteasomes.

P17980

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-20°C

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