Enzo BML-PW9300-0025 Proteasome 20S β2 subunit monoclonal antibody (MCP165) (25 µl)

Proteasome subunit β type-7, Macropain chain Z

IHC, WB

Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations.
Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue.
Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28kDa.

MCP165

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

PSMB7 (gene name)

Mouse

Dinitrophenylated proteasomes.

IgG1

Protein G affinity purified.

Western Blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Purified from hybridoma tissue culture supernatant.

Human, Mouse

Recognizes the β2 subunit of the 20S proteasome.

Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from “standard” nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.

Q99436 (human), P70195 (mouse)

This antibody is covered by our Worry-Free Guarantee.

Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10μl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.

Avoid freeze/thaw cycles.

-20°C

Blue Ice