Enzo BML-SA3400-0100 α-Synuclein polyclonal antibody (100 µl)

NACP, Non-A β component of senile plaques

IHC, WB

Western blot: The antibody has been characterized by single dimension SDS-PAGE using recombinant α- and β-synucleins as well as rat, mouse and human brain lysates with and without peptide blockade. Studies with the recombinant proteins demonstrated the absolute specificity of the antibody for α-synuclein. The antibody gives a strong band at a relative molecular weight of approximately 15kDa with human brain lysate which is wholly abolished by preincubation with the immunizing peptide (Prod. No. BML-SP9340).

Immunohistochemistry: The antibody has been tested on de-waxed tissue sections of human brain fixed in neutral buffered formaldehyde. A standard protocol should be followed.

Does not cross-react with mouse α-synuclein.

Liquid. In PBS, pH 7.4, containing 10mM sodium azide.

2136215

Rabbit

Synthetic peptide corresponding to [Cys] + aa 117-131 of human α-synuclein conjugated to KLH. The sequence is conserved in rat α-synuclein except for two substitutions, namely Asp121Ser and Asn122Ser.

Purified by caprylic acid and ammonium sulphate precipitation.

Western blot (1:1,000-1:20,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Purified from rabbit serum.

Human, Rat

This antibody has been shown to react strongly with human brain-derived α-synuclein. The antibody also reacts with rat brain-derived α-synuclein but reactivity appears substantially less than with human.

P37840

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Vial contents, Storage and Use: Vial contains an immunoglobulin preparation, partially purified by chromatography from whole serum, suspended in phosphate-buffered saline (pH 7.4) containing 0.01M sodium azide. Store unopened vial at -20°C until required for use. AVOID REPEATED FREEZE-THAW CYCLES. Aliquot undiluted antibody into smaller volumes (not less than 10μL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

-20°C

Blue Ice