Enzo BML-SE268-0010 ADAM17 (catalytic domain) (human), (recombinant) (His-tag) (10 µg)

TACE, A disintegrin and metalloproteinase 17, Tumor necrosis factor-α-converting enzyme

Useful tool to study enzyme kinetics, cleave target substrates, screen inhibitors.

Liquid. In 22.5mM TRIS, pH 7.5, containing 4.5µM ZnCl₂, 0.0045% Brij-35 and 10% glycerol.

~30.5kDa (calculated), ~36kDa doublet (SDS-PAGE)

≥90% (SDS-PAGE)

Purified by multi-step chromatography.

Recombinant glycosylated catalytic domain (aa Pro¹⁸-Val⁴⁷⁷) of ADAM17/TACE (A disintegrin and metalloproteinase 17; Tumor necrosis factor-α-converting enzyme), cloned from human cDNA (NM_003183), secreted as mature, active enzyme from insect cells, and purified using a C-terminal His-tag.

Produced in insect cells. Produced in a baculovirus expression system.

≥1800 U/µg enzyme. One unit will hydrolyze one pmole Mca-PLAQAV-Dpa-RSSSR-NH₂ substrate (Prod. No. BML-P132) (10µM) per minute at 37°C, in 25mM TRIS, pH 9.0.

P78536

Salts (sodium chloride, calcium chloride, etc.) in the assay are inhibitory. ADAM17/TACE is stable after 6 freeze-thaws at ~0.4µg/µl; freeze-thaw stability of more dilute preparations has not been tested and could lead to loss of activity.

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

-80°C

Dry Ice