Enzo BML-SE284-0100 Neutrophil elastase (human), (purified) (100 µg)

Leukocyte elastase, LE

Useful for inhibitor screening, kinetic studies, and cleavage of substrates in vitro or in tissue culture.

≥95% (SDS-PAGE). Although this is in highly purified form, and the source material tested negative for HbsAg, anti-HBc, anti-HCV, and anti-HIV-1 and -2, handle this product with all due precautions.

Reconstitute with 50mM sodium acetate, pH 5.5, with 150mM sodium chloride to the original volume, followed by gentle mixing to ensure adequate homogenization.

Isolated from human neutrophils. Active enzyme.

20-22 U/mg protein. One unit will hydrolyze 1.0 μmole of MeOSuc-Ala-Ala-Pro-Val-pNA (Prod. No. BML-P213) per minute, at 25°C in 100mM Tris-HCl, pH 7.5 with 500 mM NaCl.

P08246

As indicated on product label or CoA when stored as recommended. This enzyme is stable when stored under the above conditions. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity.

Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

-20°C

Dry Ice