Enzo BML-SE533-0050 HDAC2 (full-length) (human), (recombinant) (His-tag) (50 µg)

Histone deacetylase 2

Liquid. In 50mM TRIS, pH 8.0, 138 mM NaCl and 10% glycerol.

~66 kDa

Partially purified by single-step affinity chromatography and gel filtration.

HDAC2 from human cDNA. Sequence is identical to residues 1-582 at Genbank accession# NM_00157.2.

Produced in insect cells. Produced in a baculovirus expression system.

≥0.5 U/µg. One U=1 pmol/min at 37°C, 100 µM, FLUOR DE LYS^®-SIRT1 substrate (p53 K(Ac)-382 fluorogenic substrate, Prod. No. BML-KI177).

Q92769

The enzyme is stable on ice for the time typically required to set up an experiment (30-60 min.), but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.

-80°C

Dry Ice