Enzo BML-SE535-0100 Tyrosinase (human), (recombinant) (His-tag) (100 µg)

One microgram of BML-SE535 will produce greater than 90pmol of adduct at 37°C over a period of 20 minutes using 0.5mM L-Tyrosine as substrate in 50mM potassium phosphate, pH 7.3, 2% dimethylformamide, 5mM MBTH (3-methyl-2-benzothiazolinone hydrazone) and monitoring formation of dopachrome-MBTH adduct at 484nm (ε484=22,300 M-1cm-1). NOTE: tyrosinases, in particular mammalian tyrosinase, are inherently inefficient enzymes, with typical changes in OD only in the deciOD range, although the reaction reaches maximal velocity quickly (typically within two minutes). In addition, its substrates and MBTH are very unstable; make stocks fresh and keep sealed in the dark until use. Lifetime and rate of enzymatic activity can be increased by the addition of CuCl to the assay buffer, but this will increase background and may complicate other redox reactions in the assay.

Monophenol monooxygenase

Useful tool to study enzyme kinetics and screen for inhibitors.

Liquid. In 50mM TRIS/HCl, pH 8.0, 1M NaCl, 0.05% Empigen BB, and 30% glycerol.

~52kDa (SDS-PAGE)

≥85% (SDS-PAGE)

Purified by multi-step chromatography.

Produced in E. coli.

P14679

Avoid freeze/thaw cycles.

-80°C

Dry Ice