Enzo BML-SE555-0050 SIRT5 (human), (recombinant) (His-tag) (50 kU)

Sirtuin 5, Sir2-like protein 5, SIR2L5, NAD-dependent deacetylase Sirtuin-5

Useful for studies of enzyme kinetics, modulator activity, drug discovery.

Liquid. In 25mM TRIS, pH 7.5, 100mM sodium chloride, 5 mM DTT, containing 10% (v/v) glycerol.

~32.3kDa

≥95% (SDS-PAGE)

Purified by multi-step chromatography.

Produced in E. coli.

One unit = 1 pmol/min at 37°C, 250µM FLUOR DE LYS^® Succinyl, Desuccinylase Substrate (Prod. No. BML-KI590), 2mM NAD⁺.

Q9NXA8

The enzyme is stable on ice for the time typically required to set up an experiment (30-60 min.), but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the SIRT5 into separate tubes and store at -80° C.NOTE: When stored under the above conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing, could lead to loss of activity.

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

-80°C

Dry Ice