Mfg Part Number
BML-SE556-0050

Enzo BML-SE556-0050 PRL-1 (human), (recombinant) (His-tag) (50 µg)

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Enzo BML-SE556-0050 PRL-1 (human), (recombinant) (His-tag) (50 µg)
$627.12
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Alternative Name

Phosphatase of regenerating liver 1, Protein tyrosine phosphatase type IVA 1

Application Notes

Useful for studies of enzyme kinetics and regulation, dephosphorylation of target substrates, and inhibitor screening.

Formulation

Liquid. In 40mM TRIS-HCl, pH 8.0, containing 110mM sodium chloride, potassium chloride, 200mM imidazole, 20% glycerol and 3mM DTT.

MW

~19.8kDa

Purity

≥75% (SDS-PAGE)

Source

Produced in E. coli. The catalytic domain of PRL-1 (aa 2-173) is fused at the N-terminus to a His-tag.

Specific Activity

≥0.168U/min/μg. One unit will hydrolyze 1pmol para-nitrophenyl phosphate (PNPP) per minute at 37°C. Assay buffer: 50mM TRIS, pH 7.4, containing 150mM sodium chloride, 5mM dithiothreitol and 12.5mM PNPP.

UniProt ID

Q93096

Use/Stability

Dilution of the enzyme followed by refreezing may lead to loss of activity.

Long Term Storage

-80°C

Shipping

Dry Ice

More Information
Brand Enzo
UNSPSC 12352202