Enzo BML-SE561-0010 MMP-19 (catalytic domain) (human), (recombinant) (10 µg)

Preincubation of MMP-19 catalytic domain at 1.9µM with the broad-spectrum inhibitor GM6001 (Prod No. BML-EI300) at 100nM for 30 minutes only weakly inhibits enzymatic activity (27%).

Matrix metalloproteinase 19, RASI

Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.

Liquid. In 25mM NaBorate, pH 9.3, containing 5mM CaCl₂, 20μM ZnCl₂, 0.05% Brij-35 and 20% glycerol.

19.2 kDa

≥95% (SDS-PAGE)

Purified by multi-step chromatography.

Recombinant MMP-19 corresponding to aa Leu⁹⁹-Ser²⁵⁹ (NM_002429), which comprises the catalytic domain of human MMP-19, with a C-terminal purification tag.

Produced in E.coli. Active recombinant MMP-19.

≥ 0.40U/µg. One U=100 pmol/min at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100µM; Prod. No. BML-P125) as substrate.

Q99542

When stored under the recommended conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution followed by refreezing could lead to loss of activity.

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

-80°C

Dry Ice