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Mfg Part Number

BML-UW0200-0025

Enzo BML-UW0200-0025 Hdm2 (catalytic RING domain) (human), (recombinant) (GST-tag) (25 µg)

ENZO

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Quick Overview

Enzo BML-UW0200-0025 Hdm2 (catalytic RING domain) (human), (recombinant) (GST-tag) (25 µg)

$242.32

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Description

Activity
Modifies p53 (Prod. No. BML-FW8820) in ubiquitinylation reactions containing Ube1 (Prod. No. BML-UW9410), UbcH5b (Prod. No. BML-UW9060) and energy regeneration solution.
Alternative Name
Human double minute 2 protein
Application Notes
Uses:
1.in vitro generation of ubiquitinylated p53 for use in Ub-p53 related studies.
2.Investigation of the p53 ubiquitin modification process itself.
3.Ubiquitin modification of other known Hdm2 substrate proteins.
4.NEDD8 modification of p53.
Formulation
Liquid. In TBS, pH 7.5, containing 150mM sodium chloride and 1mM DTT.
MW
~35kDa
Purity
≥95% (SDS-PAGE)
Purity Detail
Purified by glutathione affinity chromatography.
Source
Produced in E. coli BL21 (λDE3).
UniProt ID
Q00987
Long Term Storage
-80°C
Shipping
Dry Ice

Activity	Modifies p53 (Prod. No. BML-FW8820) in ubiquitinylation reactions containing Ube1 (Prod. No. BML-UW9410), UbcH5b (Prod. No. BML-UW9060) and energy regeneration solution.
Alternative Name	Human double minute 2 protein
Application Notes	Uses: 1.in vitro generation of ubiquitinylated p53 for use in Ub-p53 related studies. 2.Investigation of the p53 ubiquitin modification process itself. 3.Ubiquitin modification of other known Hdm2 substrate proteins. 4.NEDD8 modification of p53.
Formulation	Liquid. In TBS, pH 7.5, containing 150mM sodium chloride and 1mM DTT.
MW	~35kDa
Purity	≥95% (SDS-PAGE)
Purity Detail	Purified by glutathione affinity chromatography.
Source	Produced in E. coli BL21 (λDE3).
UniProt ID	Q00987
Long Term Storage	-80°C
Shipping	Dry Ice

Specifications

More Information
Brand	Enzo
UNSPSC	12352202

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