Enzo BML-UW0500-1000 SUMO-1 (93-97)-AMCA (1 mg)

Useful fluorogenic substrate for deSUMOylating enzymes.

Typical assay conditions:
Assay substrate concentration: 12.5µM-100µM.
Enzyme concentrations: 0.5µM-4µM.
Release of AMCA fluorescence by deSUMOylating enzymes can be monitored using fluorimetry (380nm excitation and 460nm emission wavelengths).

Uses:

1. Substrate for deSUMOylating enzyme activity assays.
2. Identification/confirmation of enzyme deSUMOylation activity.
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC/AMCA substrates (e.g. SUMO1-AMC, NEDD8-AMCA).

Lyophilized solid.

747

≥95% (HPLC)

E⁹³QTGG⁹⁷-AMCA

Soluble in DMSO. Can be subsequently diluted in aqueous buffers.

As indicated on product label or CoA when stored as recommended. Store solid at -20°C for up to twelve months. Solutions can be stored for up to three months at -20°C.

Avoid freeze/thaw cycles.

-20°C

Blue Ice