Enzo BML-UW0730-0050 Di-ubiquitin (K63-linked) (50 µg)

Ub2

For use in deubiquitinylating enzyme assays and polyubiquitin binding studies.

Suggested uses:

1. Deubiquitinylating enzyme substrates (general/linkage specific).
2. Investigation of polyubiquitin chain recognition by and interaction with ubiquitin binding proteins.

Liquid. In 20mM TRIS-HCl, pH 7.5, containing 0.15M NaCl, 1mM EDTA.

~17kDa

≥95% (SDS-PAGE)

Protein components produced in E. coli. Synthesised enzymatically in vitro.

K⁶³-linked di-ubiquitin (Ub₂) is made by incubating E1 and Ubc13-Mms2 with equal concentrations of ubiquitin capped at the future proximal chain terminus (Asp⁷⁷-ubiquitin) and ubiquitin capped at the future distal chain terminus (K⁴⁸R/K⁶³R-ubiquitin). The distal terminus of the resulting dimer is de-blocked by treatment with yeast ubiquitin hydrolase-1 (YUH-1) followed by purification by anion exchange chromatography.

P0CG47 (UBB), P0CG48 (UBC)

As indicated on product label or CoA when stored as recommended. Stable for at least 12 months after receipt when stored at -20°C. It is recommended that the product be kept at a moderately high protein concentration (>1mg/mL) and in the presence of buffer. If a more dilute reagent is required, the inclusion of an inert carrier protein (e.g. ovalbumin at 0.5-1.0mg/mL) in the storage buffer is recommended in order to minimise loss of the reagent due to absorption.

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

-20°C

-20°C

Blue Ice