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Mfg Part Number

BML-UW9060-1000

Enzo BML-UW9060-1000 UbcH5b (human), (recombinant) (His-tag) (1 mg)

ENZO

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Enzo BML-UW9060-1000 UbcH5b (human), (recombinant) (His-tag) (1 mg)

$3,184.48

Order will ship within 1-3 business days

Description

Application Notes
Useful for in vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions.
Biological Activity
The His6-tagged fusion proteins of UbcH5a, UbcH5b and UbcH5c all charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The [C⁸⁵A] mutation (Prod. No. BML-UW9065) completely abolishes the ability of the enzyme to form a thiol ester and thereby acts as an excellent negative control.
Formulation
Liquid. In 25mM TRIS-HCl, pH 7.5, containing 150mM NaCl and 0.5mM DTT.
MW
~18kDa
Purity
≥90% (SDS-PAGE)
Source
Produced in E. coli.
UniProt ID
P62837
Use/Stability
Enzyme is stable to multiple freeze/thaw cycles.
Long Term Storage
-80°C
Shipping
Dry Ice

Application Notes	Useful for in vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions.
Biological Activity	The His6-tagged fusion proteins of UbcH5a, UbcH5b and UbcH5c all charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The [C⁸⁵A] mutation (Prod. No. BML-UW9065) completely abolishes the ability of the enzyme to form a thiol ester and thereby acts as an excellent negative control.
Formulation	Liquid. In 25mM TRIS-HCl, pH 7.5, containing 150mM NaCl and 0.5mM DTT.
MW	~18kDa
Purity	≥90% (SDS-PAGE)
Source	Produced in E. coli.
UniProt ID	P62837
Use/Stability	Enzyme is stable to multiple freeze/thaw cycles.
Long Term Storage	-80°C
Shipping	Dry Ice

Specifications

More Information
Brand	Enzo
UNSPSC	12352202

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