JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

BML-UW9195-0500

Enzo BML-UW9195-0500 SUMO-1 (human), (recombinant) (His-tag) (500 µg)

ENZO

Be the first to review this product

Quick Overview

Enzo BML-UW9195-0500 SUMO-1 (human), (recombinant) (His-tag) (500 µg)

$273.52

Order will ship within 1-3 business days

Description

Alternative Name
Sentrin-1, Small ubiquitin-related modifier 1
Application Notes
In the presence of HeLa Fraction II or a combination of SUMO E1 and E2 (Prod. No. BML-UW9330 and BML-UW9320 respectively), with ATP, Mg²⁺, and DTT, mature SUMO-1 may be conjugated to soluble proteins. Typical in vitro concentration for non-rate limiting conjugate formation is 200μM to 1mM, depending upon conditions.
Uses:
1. SUMO-1 modification of specific proteins in vitro.
2. Demonstrate novel proteins are potential targets for SUMOylation under in vitro conditions.
3. Generate substrates for deSUMOylating enzymes.
Formulation
Liquid. In HEPES, pH 8.0, containing 50mM NaCl, 1mM DTT.
MW
~12kDa
Purity
≥95% (SDS-PAGE)
Source
Produced in E. coli. Human SUMO-1 is fused to a His-tag.
UniProt ID
P63165
Handling
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Long Term Storage
-80°C
Shipping
Dry Ice

Alternative Name	Sentrin-1, Small ubiquitin-related modifier 1
Application Notes	In the presence of HeLa Fraction II or a combination of SUMO E1 and E2 (Prod. No. BML-UW9330 and BML-UW9320 respectively), with ATP, Mg²⁺, and DTT, mature SUMO-1 may be conjugated to soluble proteins. Typical in vitro concentration for non-rate limiting conjugate formation is 200μM to 1mM, depending upon conditions. Uses: 1. SUMO-1 modification of specific proteins in vitro. 2. Demonstrate novel proteins are potential targets for SUMOylation under in vitro conditions. 3. Generate substrates for deSUMOylating enzymes.
Formulation	Liquid. In HEPES, pH 8.0, containing 50mM NaCl, 1mM DTT.
MW	~12kDa
Purity	≥95% (SDS-PAGE)
Source	Produced in E. coli. Human SUMO-1 is fused to a His-tag.
UniProt ID	P63165
Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Long Term Storage	-80°C
Shipping	Dry Ice

Specifications

More Information
Brand	Enzo
UNSPSC	12352202

Reviews