Enzo ENZ-42420-10 BIOARRAY™ Single-round RNA amplification and biotin labeling system (10 Reactions)

Box A:
40 µl, dNTP Mix (dN)
10 µl, Promoter Primer (P)
20 µl, First Strand Buffer (FB)
80 µl, DTT (D)
10 µl, Reverse Transcriptase (RT)
10 µl, RNase Inhibitor (I)
50 µl, DNA Polymerase (DP)
10 µl, RNase H (RH)
150 µl, Second Strand Buffer (SB)
60 µl, IVT Reaction Buffer (RB)
60 µl, 10x Biotin-Labeled Ribonucleotides (B)
60 µl, Enhancer Cocktail (EC)
30 µl, T7 RNA Polymerase (T7)
2.5 mL, Nuclease-free Water (W)
Box B:
4.5 ml, cDNA Binding Buffer (BB)
1.2 ml, 5X Wash Solution (WS)
10 cDNA Purification Columns
10 cDNA Elution Tubes
10 Collection Tubes

• aRNA yield is ≥23µg
• aRNA purity range is between an A₂₆₀/A₂₈₀ ratio of 1.9 and 2.3
• Median aRNA size is equal to or greater than 1200 nt
• Affymetrix GeneChip criteria:

• % Present calls ≥ than 40%
• Scale factors ≤ 3.0
• 5’/3’ ratios for actin and GAPDH ~ 1.0 ± 0.3

As indicated on product label or CoA when stored as recommended. Store Box A at -20˚C in a non-frost free freezer. Store Box B at ambient condition.

Avoid freeze/thaw cycles.

-20°C

Dry Ice