Enzo ENZ-51010 ROS-ID® Total ROS/Superoxide detection kit (1 Kit)

Reactive oxygen species / Superoxide

Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.

Oxidative Stress Detection Reagent (Green), 300 nmoles
Superoxide Detection Reagent (Orange), 300 nmoles 
ROS Inducer (Pyocyanin), 1 µmole
ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg
Wash Buffer Salts, 1 pack

A sample from each lot of ROS-ID^® Total ROS/Superoxide detection kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard green (490/525 nm) and orange (550/620 nm) filter set.
The following results are obtained: ROS positive control samples induced with Pyocyanin exhibit bright orange signal in nucleus as well as bright green fluorescence in the cytoplasm. Cells pretreated with the ROS inhibitor don’t demonstrate any green or orange fluorescence signal upon induction.

200 fluorescence microscopy assays or 50 flow cytometry assays or 2×96 microplate assays.

The ROS-ID^® Total ROS/Superoxide detection kit is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLELSTIAL^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Toxicology Application Note:
Use of 3D Cultured Human iPSC-Derived Hepatocytes for Long-Term Hepatotoxicity Studies
Screening Reactive Oxygen Species (ROS) on IQUE® Screener

Cited samples:
For an overview on cited samples please click here.

With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

Protect from light. Avoid freeze/thaw cycles.

-20°C

-80°C

Dry Ice