Enzo ENZ-51014-100 NUCLEAR-ID® Green cell cycle kit (1 Kit)

Flow Cytometry, Fluorescence microscopy

This kit is suitable for DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels.

NUCLEAR-ID^® Green Cell Cycle Detection Reagent, 100 µl
Nocodazole Control, 10 µl
10X Assay Buffer, 15 ml

1. Absorption peak of NUCLEAR-ID^® Green dye: λ_max = 507 ± 4 nm

% purity of NUCLEAR-ID^® Green dye by HPLC: ≥93%

A sample from each lot of NUCLEAR-ID^® Green Cell Cycle Analysis Kit is used to analyze Jurkat cells using the procedures described in the user manual. Cells with Nocodazole gave %G₂ value of >50%. Untreated cells gave the following results: (a) G₀/G₁ peak CV < 15%; (b) %G₁ > 35%; (c) %G₂ < 30%; and (d) G₂/G₁ ratio > 1.72.

100 assays

The NUCLEAR-ID^® Green cell cycle analysis kit is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required .

Protect from light. Avoid freeze/thaw cycles.

-20°C

-80°C

Dry Ice