Enzo ENZ-51015-KP002 LYSO-ID® Red cytotoxicity kit (GFP-CERTIFIED®) (1 Kit)

Fluorescence microscopy, Fluorescent detection

96-well cell-based assay. Provides a rapid and quantitative approach for determining drug- or toxic agent-induced lysosome and lysosome-like organelle perturbations in live cells.

10x dual color detection reagent: 2 x 1ml
Detection buffer: 20ml
Verapamil control: 3μmoles
10x assay buffer: 15ml

A sample from each lot of LYSO-ID^® Red cytotoxicity kit (GFP-CERTIFIED^®) is used to stain U2OS, epithelial cell line (MDCK) and HeLa cells, using the procedures described in the user manual. Cells treated with verapamil showed significant increase in red lysosome signaling and no significant loss of blue nucleus counterstaining compared to the control cells.

2 x 96-well plates

With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

Protect from light. Avoid freeze/thaw cycles.

-80°C

Dry Ice