Enzo ENZ-51021-K200 NUCLEAR-ID® Green chromatin condensation detection kit (1 Kit)

Flow Cytometry, Fluorescence microscopy, Fluorescent detection

This kit provides a rapid assay for nuclear condensation, a prominent hallmark of apoptosis.

NUCLEAR-ID^® Green Cell Cycle Detection Reagent, 100 µl Apoptosis inducer (Staurosporine), 50nmoles 10X Assay Buffer, 30 ml

Absorption peak of NUCLEAR-ID^® Green dye: λ_max = 507 ± 4 nm

% purity of NUCLEAR-ID^® Green dye by HPLC: ≥93% 

A sample from each lot of NUCLEAR-ID^® Green Cell Cycle Analysis Kit is used to analyze Jurkat cells using the procedures described in the user manual. The following results are obtained:
(a) Untreated control cells: Viable cells: >85%; Apoptotic cell population: <15%
(b) Apoptosis inducer treated cells: Viable cells: >60%; Apoptotic cell population: <30%
(c) Mean fluorescence of apoptotic cells / mean fluorescence of viable cells: >40

200 assays

Protect from light. Avoid freeze/thaw cycles.

-20°C

-80°C

Dry Ice