Enzo ENZ-51031-0050 CYTO-ID® Autophagy detection kit (50 tests)

Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

The CYTO-ID^® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy, flow cytometry, and microplate reader.

CYTO-ID^® Green Detection Reagent
Hoechst 33342 Nuclear Stain
Autophagy Inducer (Rapamycin)
Chloroquine Control
10X Assay Buffer

A sample from each lot of CYTO-ID^® Autophagy detection kit is used to stain HeLa Cells as described in user manual. CYTO-ID^® autophagy detection reagent is incorporated into induced cells, observed as accumulative typical spherical vacuoles in foci or throughout cytoplasm. Comparing to untreated HeLa cells, treated sample demonstrate significant increase in fluorescence under microscope.

For -K200 size:
200 flow cytometry assays, 250 microscopy assays or 3 x 96-well microplate assays.

For -0050 size:
50 flow cytometry assays, 60 microscopy assays or 1 x 96-well microplate assays.

The CYTO-ID^® Autophagy Detection kit is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications.

Featured in:
Genetic Engineering & Biotechnology News – HTS Profiling Method for Autophagy-Modulators

Visualizing subcellular vesicles to quantitate autophagy in neuronal cells

Cited samples:
For an overview on cited samples please click here.

With proper storage, the kit components are stable for one year from date of receipt.

Protect from light. Avoid freeze/thaw cycles.

-20°C

-80°C

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