Enzo ENZ-51035-K100 PROTEOSTAT® Aggresome detection kit (100 tests)

Flow Cytometry, Fluorescence microscopy, Fluorescent detection

The PROTEOSTAT^® Aggresome detection kit assay is potentially applicable to the identification of small molecules that inhibit aggresome formation as well as immuno-localization studies between aggregated protein cargo and the various proteins implicated in aggresome formation, such as histone deacetylase 6, parkin, ataxin-3, dynein motor complex and ubiquilin-1.

PROTEOSTAT^®Aggresome detection reagent
Hoechst 33342 Nuclear stain
Proteasome Inhibitor (MG-132)
10X Assay Buffer

A sample from each lot of PROTEOSTAT^® Aggresome detection kit is used to stain Jurkat cells and analyzed by flow cytometry, using the procedures described in the user manual. The aggresome activity factor (AAF) values for the samples are greater than 25.

For ENZ-51035-0025:
25 flow cytometry assays or 50 microscopy assays

For ENZ-51035-K100:
100 flow cytometry assays or 200 microscopy assays

The PROTEOSTAT^® Aggresome Detection Kit is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Featured In:
High-throughput image-based aggresome quantification (SLAS Discov 2020; 25:783-791).
Application: Optimized to monitor aggresome formation in a miniaturized, automated, and quantitative phenotypic assay. The approach is validated by screening a chemical library of 1280 compounds.

Application Note:
A Red-emitting Fluorescent Probe for Rapid Detection of Protein and Peptide Aggregates in Post-mortem Human Brain Tissue Sections from Patients Diagnosed with Alzheimer’s and Parkinson’s Disease

Monitoring the Accumulation and Clearance of Exogenously Introduced Beta-amyloid in a Cell-Based Model of Alzheimer’s Disease by Fluorescence Microscopy and Fluorescence Microplate Assay

Towards Understanding the Molecular Basis of Parkinson’s Disease: Cell-based Model of Mitophagy and Aggresome Accumulation

Detection of bacterial aggregation by flow cytometry

Cell-Based Screening of Focused Bioactive Compound Libraries: Assessing Small Molecule Modulators of the Canonical Wnt Signaling and Autophagy-Lysosome Pathways

Monitoring Whole Brain Plaque Burden with PROTEOSTAT® Aggresome Detection Kit and Light Sheet Microscopy

Cited samples:
For an overview on cited samples please click here.

Enzo and PROTEOSTAT^® are trademarks of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.

With proper storage, the kit components are stable for one year from date of receipt.

Protect from light. Avoid freeze/thaw cycles.

-20°C

-80°C

Blue Ice