Enzo ENZ-60002-C005 Dkk-1 (mouse), (recombinant) (active) (5 µg)
| Alternative Name | Dickkopf-related protein-1 |
|---|---|
| Biological Activity | Determined measuring the ability to inhibit the induction of the LEADING LIGHT® Wnt Reporter Cell Line (Prod. No. ENZ-61002) induced for at least 6 hours with 200 ng/ml Wnt3a (Prod. No. ENZ-60001). The EC50 of this inhibition is 45-55 ng/ml. |
| Endotoxin Content | <1.0EU/µg (LAL method) |
| Formulation | Lyophilized from a solution containing 1X PBS, 1% CHAPS, and 0.1% BSA. |
| MW | ~40kDa (SDS-PAGE) |
| Purity | ≥80% (SDS-PAGE) |
| Quantity | 5µg |
| Reconstitution | Reconstitute by adding 1.5ml water to make a 3.3µg/ml solution. |
| Sequence | Mouse Dkk-1 amino acids 33-272, as well as a TEV recognition site and 6 histidines at the C terminus. |
| Source | Produced in insect cells. Produced in a baculovirus expression system. |
| UniProt ID | O54908 |
| Use/Stability | Stable for at least 6 months after receipt when stored as supplied at -80°C. |
| Handling | Avoid freeze/thaw cycles. |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Shipping | Dry Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352202 |