Enzo ENZ-C34933 Smooth muscle actin monoclonal antibody (CGA7) (0.5 ml)
| Application | IHC (PS), WB |
|---|---|
| Clone | CGA7 |
| Crossreactivity | Does not cross-react with actin of skeletal or cardiac muscle cells. |
| Formulation | Liquid. In PBS containing 0.1% sodium azide and 1% BSA. |
| Host | Mouse |
| Immunogen | Partially purified chicken gizzard actin. |
| Isotype | IgG2b |
| Purity Detail | Affinity purified. |
| Recommendation Dilutions/Conditions | Immunohistochemistry (paraffin sections; optimal results with this antibody are obtained when the tissue to be stained is fixed in alcohol-based fixatives such as methacarn. Aldehyde-fixed (formalin, e.g.) specimens are not recommended for use with CGA7. Choose a good quality enzyme-linked anti-mouse IgG2b antibody for detection).General suggested starting dilution is 1:20-1:50.Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Source | Purified from hybridoma tissue culture supernatant. |
| Species Reactivity | Chicken, Human, Monkey, Rabbit |
| Specificity | Recognizes actin isotypes α and γ of smooth muscle cells. This antibody labels leiomyosarcomas and leiomyomas but does not react with other neoplasms such as carcinoma or lymphoma. Except for myoepithelium, it is nonreactive with other mesenchymal and all epithelial cells. |
| Worry-free Guarantee | This antibody is covered by our Worry-Free Guarantee. |
| Handling | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Shipping | Blue Ice |
| Brand | Enzo |
|---|---|
| UNSPSC | 12352203 |