Enzo ENZ-GEN505-0012 AMPINEXT™ High-Sensitivity DNA Library Preparation Kit (Illumina) (12 Reactions)

A complete set of optimized reagents to prepare a DNA library from very small amount of samples for use in next-generation sequencing applications.

1 hour 30 minutes

10X End Polishing Buffer
End Polishing Enzyme Mix
End Polishing Enhancer
2X Ligation Buffer
T4 DNA Ligase
Adaptors (50 µM)
MQ Binding Beads
2X HiFi PCR Master Mix
Primer U (10 µM)
Primer I (10 µM)
Elution Buffer

Starting materials can include fragmented dsDNA isolated from various tissue or cell samples, dsDNA enriched from a ChIP reaction, MeDIP/hMeDIP reaction, or exon capture. DNA should be relatively free of RNA because large fractions of RNA will impair end repair and dA-tailing, resulting in reduced ligation capabilities. The input amount of DNA can be from 0.2 ng to 100 ng. For optimal preparation, the input amount should be 10 ng to 50 ng.

Upon receipt: Store the following components at -20°C immediately: 10X End Polishing Buffer, End Polishing Enzyme Mix, End Polishing Enhancer, 2X Ligation Buffer, T4 DNA Ligase, Adaptors, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. Store the following components at 4°C: MQ Binding Beads. Store all other components at room temperature. ​

Blue Ice