Enzo ENZ-GEN510-0012 AMPINEXT™ RNA Bisulfite-Seq Kit (Illumina) (12 Reactions)

A complete set of optimized reagents designed to carry out RNA bisulfite conversion, followed by a “post-bisulfite” library preparation process for Illumina platform-based bisulfite sequencing, all in one kit.

6 hours

Conversion Buffer
Conversion Powder
NA Binding Solution
F-Spin Column
F-Collection Tube
Desulphonation Solution
5X RT Reaction Buffer
10 mM dNTP Mix
0.1M DTT
RNase Inhibitor
Random Primer (50 µM)
RT Enzyme Mix
10X End Polishing Buffer
End Polishing Enzyme Mix
End Polishing Enhancer
2X Ligation Buffer
T4 DNA Ligase
Adaptors (50 µM)
MQ Binding Beads
2X HiFi PCR Master Mix
Primer U (10 µM)
Primer I (10 µM)
Elution Buffer

Upon receipt: (1) Store the following components at -20°C immediately: 5X RT Reaction Buffer, 10 mM dNTP mix, 0.1M DTT, RNase Inhibitor, Random Primer, RT Enzyme Mix, 10X End Polishing Buffer, End Polishing Enzyme Mix, End Polishing Enhancer, 2X Ligation Buffer, T4 DNA Ligase, Adaptors, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. Store the following components at 4°C: MQ Binding Beads. Store all other components at room temperature.

Blue Ice