Enzo ENZ-GEN512-0012 AMPINEXT™ Post-Bisulfite DNA Library Preparation Kit (Illumina) (12 Reactions)

A complete set of optimized reagents to prepare a DNA library, after successful bisulfite conversion, for various Illumina platform-based bisulfite sequencing (bisulfite-seq) assays.

5 hours

5X Conversion Buffer
Conversion Enzyme Mix
Conversion Primer
10X End Repair Buffer
End Repair Enzyme Mix
10X dA-Tailing Buffer
Klenow Fragment (3’-5’ exo^–)
2X Ligation Buffer
T4 DNA Ligase
Adaptors (50 µM)
MQ Binding Beads
2X HiFi PCR Master Mix
Primer U (10 µM)
Primer I (10 µM)
Elution Buffer

Upon receipt, store the following components at -20°C immediately: 5X Conversion Buffer, Conversion Enzyme Mix, Conversion Primer, 10X End Repair Buffer, End Repair Enzyme Mix,10X dA-Tailing Buffer, Klenow Fragment (3’-5’ exo-), 2X Ligation Buffer, T4 DNA Ligase, Adaptors, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. Store the following components at 4°C: MQ Binding Beads. Store all other components at room temperature. ​

+4°C

Blue Ice