Enzo JBS-EGE-1410ble LEXSinduce3 Expression Kit (contains the integrative vector pLEXSY_I-ble3) (1 Kit)

• LEXSY host T7-TR: kit contains three vials with 1.6 ml each of frozen glycerol stocks of LEXSY host T7-TR.
• pLEXSY_I-3 expression vector pLEXSY_I-blecherry3 (Cat. No. JBS-EGE-243), 5 µg in 10 mM Tris HCl pH 8.0
• pLEXSY_I-3 control plasmid with EGFP gene pLEXSY_I-egfp-ble3 (Cat. No. JBS-EGE-247), 5 µg in 10 mM Tris HCl pH 8.0
• Primers for diagnostic PCR and sequencing:

ble forward primer A708, for pLEXSY_I-ble3 and pLEXSY_I-blecherry3
Insert sequencing forward primer P1442
Insert sequencing reverse primer A264
5`utr (aprt) reverse primer A1715
odc forward primer A1304
odc reverse primer P1510

• LEXSY BHI, Powder for preparation of liquid cultivation medium
• LEXSY Bleo for pLEXSY_I-ble3 and pLEXSY_I-blecherry3 – 1 ml, ready-to-use 1000x stock solution, 100 mg/ml, filter-sterilized
• inducer of the T7-TR system: LEXSY Tet (Tetracycline) – 1 ml, ready-to-use 1000x stock solution, 10 mg/ml, filter sterilized
• Hemin (0.25% porcine Hemin in 50% Triethanolamine) – 2 ml, ready-to-use 500x stock solution, steril-filtered
• Pen-Strep, ready-to-use 200x stock solution, steril-filtered

For the Original Manufacturer’s data sheet please click here.

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