Enzo NZY-T035-150 Human keratinocyte transglutaminase (TG1) (150 µg)

Add 10mM Ca2+ to activate TG1.> 8,000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG1-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231). 1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex =332nm, λem =500nm; band filter =5nm; detector strength =600V; temperature =37°C, assay volume =1ml)].

Keratinocyte Transglutaminase 1, TG1, TGase 1, Keratinocyte protein-glutamine-γ-glutamyltransferase

White solid.

Lyophilized from 50mM Tris-HCl, pH 8.0, containing 10mM Glutathione.

~90kDa

≥90% (SDS-PAGE)

Purified by Ni2+-affinity chromatography.

Reconstitute with water. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage.

Based on the TGM1-allele from I.M.A.G.E.-clone IRAKp961M1628 isolated from human skin squamous cell carcinoma. It is N-terminally fused to His₆-tag.

Produced in insect cells.

For the Original Manufacturer’s data sheet please click here.

After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

-20°C

Ambient Temperature