Enzo NZY-T067-001 Human tissue transglutaminase, endotoxin free (1 mg)

>1500 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231). 1U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].

Tissue-type Transglutaminase, TG2, TGase 2, Protein-glutamine-γ-glutamyltransferase

Catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e.g. the formation of ε-(γ-glutamyl)lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His₆-rhTG2 may also be used for

Liquid. In 10mM TRIS-HCl, pH 8.1, 150mM sodium chloride, 1mM EDTA , 1mM DTT and 25% Glycerol. His₆-rhTG2 is a Ca²⁺-dependent enzyme.
Activation: Add 10mM Ca²⁺ to activate His₆-rhTG2.

~78kDa

≥90% (SDS-PAGE)

Produced in insect cells.

For the Original Manufacturer’s data sheet please click here.

After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

-20°C

Blue Ice