Kit Contents: (representative of 100 assay kit size) -Supercoiled pHOT1 DNA 25µg in 100µl -Marker DNA, Linear pHOT1; 0.05µg/µl in 1X gel loading buffer -10X Topoisomerase II assay buffer components* *NEW: To improve stability, the 10X Topo II Assay buffer is provided as two components: Buffer A (no ATP) and Buffer B (ATP). The complete 10X Assay buffer is made fresh each time by combining Buffer A and B. -Sodium dodecyl sulfate (10%), 300µl -10X Gel loading buffer, 300µl (bromophenol blue, glycerol) -Control inhibitor, Etoposide (lyophilized) Prod. No. TOP-4140 -Proteinase K (500µl) at 0.5mg/ml. This is a 10X stock of Proteinase K. -Detailed instruction manual Note: Enzyme is sold separately (Prod. No. TOP-2000H)
Use/Stability
The DNAs should be stored at 4°C and the buffers stored at -20°C upon receipt. The VP-16 can be stored at ambient temperature.
